A modified method for purification of Eimeria tenella sporozoites

Enhanced egress of intracellular Eimeria tenella sporozoites by splenic lymphocytes

Partial purification and characterization of an aminopeptidase from Eimeria tenella.

Our previous investigation demonstrated the expression in Eimeria tenella sporulated oocysts of an aminopeptidase (AP) with strong homology to AP N. To further understand the role of proteases during development, we investigated the molecular and biochemical properties of E. tenella AP. Greater than 95% AP activity was present in a soluble extract during sporulation of oocysts with highest acti...

Enhanced egress of intracellular Eimeria tenella sporozoites by splenic lymphocytes from coccidian-infected chickens.

Egress, which describes the mechanism that some intracellular parasites use to exit from parasitophorous vacuoles and host cells, plays a very important role in the parasite life cycle and is central to Eimeria propagation and pathogenesis. Despite the importance of egress in the intracellular parasite's life cycle, very little information is known on this process compared to other steps, e.g.,...

Detection of Cell Mediated Immune Response in Chickens Immunized with Eimeria tenella Sporozoites

The Indian poultry sector has become a major contributor to the national economy as a result of the revolutionary and scientific approaches in avian health care management. Indian poultry industry contributes nearly Rs. 9.9 billion annually to the national economy (Mohana Subramanian et al., 2010). In India, broiler chicks are reared in deep litter system under farm condition which leads to fre...

Expression of flotillin-1 on Eimeria tenella sporozoites and its role in host cell invasion.

Lipid rafts are detergent-resistant, liquid-ordered microdomains in plasma membranes that are enriched in cholesterol and sphingolipids and involved in intracellular signal transduction, membrane trafficking, and molecular sorting. In this study, we investigated the possibility that lipid rafts on Eimeria tenella sporozoites may act as platforms for host cell invasion. Flotillin-1, a resident p...